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dc.contributor.authorTopal, Meryem
dc.description.abstractRosmarinic acid is a water-soluble ester of ca eic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) puri ed from human erythrocytes by using Sepharose-4B a nity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold puri cation of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO2 -esterase activity gave Ki values of 86.0 M and 57.0 M, respectively.en_US
dc.subjectCarbonic anhydrase I, carbonic anhydrase II, rosmarinic acid, enzyme puri cation, enzyme inhibitionen_US
dc.titleRosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitoren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.departmentMeslek Yüksekokulları, Gümüşhane Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümüen_US
dc.contributor.institutionauthorTopal, Meryem

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