Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor

Abstract

Rosmarinic acid is a water-soluble ester of ca eic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) puri ed from human erythrocytes by using Sepharose-4B a nity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold puri cation of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO2 -esterase activity gave Ki values of 86.0 M and 57.0 M, respectively.