Highly Efficient Spectrophotometric Determination of Cu2+ ion in Aqueous Medium Using a thiosemicarbazone-derivative Ligand

Abstract

A new simple spectrophotometric method for the determination of Cu2+ ion was developed using an thiosemicarbazone compound, 2-{4-[Bis(2-chloroethyl)amino]benzylidene}-N-[(4-methylthio)phenyl]hydrazinecarbothioamide (TSC). A simultaneous color change was observed (from colorless to bright yellow) by the addition of Cu2+ ion to the TSC ligand solution. The maximum absorbance of the TSC ligand measured at 366 nm was decreased by the presence of Cu2+ ion. The graphs of absorbance obtained by means of the Job's method and the molar-ratio method proposed a complex formation with a 1:2 Cu2+-TSC ligand stoichiometry. The molar-ratio method with emission measurements also confirmed the stoichiometry. The complex stability constant of TSC-Cu2+ complex (K) was evaluated to be 1.76 x 10(5). The proposed spectrophotometric method was associated with the change in absorbance at 366 nm owing to the interaction between the TSC ligand and Cu2+ ion. From the spectrophotometric titration data, it was pointed out that TSC ligand (1.5 x 10(- 5) mol L-1) selectively reacted with Cu2+ ion in DMSO/water (1:1, v/v, citrate buffer at pH = 6.0). The calibration curve for Cu2+ ion was obtained with a good linearity in the range of 0.0191-0.3241 mg L-1. The detection limit for Cu2+ ion was 0.0063 mg L-1. The proposed method was achievemently implemented in real water samples (drink water, tap water and, distilled water). Satisfactory recoveries were confirmed at three different concentrations. The method presented a relative standard deviation (RSD%) of less than 3.08%.