Secondary Metabolites of Ethanol Extracts of Pinus sylvestris Cones from Eastern Anatolia and Their Antioxidant, Cholinesterase and alpha-Glucosidase Activities

Abstract

Scots pine (Pinus sylvestris) is the most widely distributed tree species among pine species. Antiradical, antioxidant properties and inhibition properties on butyrylcholinesterase (BChE), acetylcholinesterase (AChE) and alpha-glycosidase activities of ethanol extracts of P. sylvestris were reported from Sarikamis (Kars), Gumushane and Erzurum provinces of Turkey. The cones of P. Sylvestris from Gumushane showed the highest IC50 values in both DPPH center dot (14.75 mu g/mL) and ABTS(center dot+) (12.56 mu g/mL) radical scavenging activities. An LC-HRMS method developed and the secondary metabolite composition of extracts were identified. The major compounds are determined as (+)-trans taxifolin, quercitrin, fumaric acid, (-) epicatechin, and nepetin-7-O-glucoside, apigenin-7-O-glucoside in P. sylvestris cones.