Determination of Angelica archangelica's Antioxidant Capacity and Mineral Content

Abstract

The aim of this study was to determine the mineral contents by ICP-MS and the antioxidant capacity of Angelica archangelica plant, which is not well known in the literature. For this purpose, ethyl alcohol extracts of the branches and leaves of the plant; different bioanalytical methods such as reducing capacity by Fe3+-Fe2+ transformation method, Fe3+-TPTZ reducing capacity by FRAP method, Cu2+-Cu+ reducing capacity by CUPRAC method, the ferric ions (Fe2+) chelating activity by using bipyridyl reagent, DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, DMPD (N,N-dimethyl-p-phenylenediamine), ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging activities, total antioxidant activity determination according to thiocyanate method, total phenolic and total flavonoid activity were used. As standard, BHT, BHA, alpha-Tocopherol, Trolox were used. A. archangelica branches and leaves demonstrated 88.13 % and 93.90 % inhibition of linoleic acid emulsion peroxidation at 20 mg mL(-1) concentration. Also, it was determined that both leaves and branches of A. archangelica plant were dense with P, Mg, Ca and K minerals with ICP-MS device. It is considered that the results of the manuscript will guide the antioxidant studies, medicine, cosmetics, pharmacology and food sector.