Production of structured lipid with a low omega-6/omega-3 fatty acids ratio by enzymatic interesterification

Abstract

A structured lipid (SL) constituting omega fatty acids was synthesized by using linseed and grape seed oils as substrates via a lipase-catalyzed reaction. Lipozyme (R) TL IM was used as a biocatalyst. Good quadratic models predicting the incorporation of omega fatty acids were achieved via the Response surface methodology (RSM). The optimal conditions for targeted omega-6/omega-3 fatty acid ratio (2:1) were obtained at a substrate molar ratio 1.4, time 8.4 h, and enzyme amount 6.4%. The SL contained linoleic acid (43 g 100g(-1)), which was mainly located in the sn-2 position (40 g 100g(-1)). alpha-Linoleic acid, and alpha-linolenic acid at the sn-2 position were 22 g 100g(-1), and 11 g 100g(-1), respectively. The oxidative stability of the SL, and SL with antioxidants was also investigated. The produced SL may be proposed as a source of a balanced intake of omega fatty acids and an ingredient in functional food formulations.