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dc.contributor.authorPaksoy, Z.
dc.date.accessioned2019-12-27T07:49:10Z
dc.date.available2019-12-27T07:49:10Z
dc.date.issued2018
dc.identifier.issn23043075
dc.description.abstractIn this study, the effects of 4-Vinylcyclohexene diepoxide (VCD) on the testicular gene expression in dogs were investigated. Eighteen male dogs were used as material and were randomly allocated to 3 groups. The first group (n=6) was administered sesame oil by intraperitoneal (i.p.) route once a day for 8 days, and this group was served as a control. The second group (n=6) received 80 mg/kg VCD and the third (n=6) group was administered VCD (i.p.) at a dose of 320 mg/kg once daily for 8 days. The testes were removed on following day the last VCD administration. The testes were immediately placed in the RNA stabilization solution and stored at -20°C. Later mRNAs were obtained from tissue samples. After RNA isolation, the cDNAs were synthesized. Finally, MasterMix was prepared and gene expression levels were examined at the real-time PCR. The expression of B cell lymphoma 2 (Bcl-2), tumor suppressor protein (tp53), Bax, CASP2, CASP3, CASP8, CASP9 and ?-actin were investigated in the all groups. Cross-group comparisons were made by using student t test. The tp53 gene expression in the second group was found to be higher compared to the control group. It was determined that the expression of Bcl-2 was reduced in the testes of animals treated with high-dose VCD. CASP-3 was found to be more expressed and CASP-9 was down-regulated in the third group. In addition, CASP-8 gene expression was significantly reduced in both treatment groups. As a result, it can be concluded that expression changes in the tp53, Bcl-2, CASP-3, CASP-8 and CASP-9 genes in the testes of male dogs may cause apoptotic changes with the VCD application. © 2018 IJVS.en_US
dc.description.sponsorshipI would like to thank Biologist Ismail Hakki TAS and Research Assistant Seyda Merve KARATAS. This study has been supported by Gumushane University Scientific Research Projects Coordination Department. Project Number: 16.B0121.02.02.en_US
dc.language.isoengen_US
dc.publisherMehria Publishersen_US
dc.relation.ispartofInternational Journal of Veterinary Scienceen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject4-Vinylcyclohexene diepoxide; Apoptosis; Dog; Testisen_US
dc.titleThe effects of 4-Vinylcyclohexene diepoxide on gene expression in dog testesen_US
dc.typearticleen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.description.scopuspublicationid2-s2.0-85049616110en_US
dc.department[Belirlenecek]en_US
dc.identifier.volume7en_US
dc.identifier.issue2en_US
dc.identifier.startpage60en_US
dc.contributor.institutionauthor[Belirlenecek]
dc.identifier.endpage64en_US
dc.authorscopusid36114920300


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