dc.contributor.author | Özad Düzgün, Azer | |
dc.contributor.author | Saral, Ayşegül | |
dc.date.accessioned | 2019-12-18T11:01:02Z | |
dc.date.available | 2019-12-18T11:01:02Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12440/1755 | |
dc.identifier.uri | https://www.scopus.com/record/display.uri?eid=2-s2.0-85041681727&origin=resultslist&sort=plf-f&src=s&st1=Molecular+docking+analysis+and+determination+of+minimum+inhibition+concentration+of+VIM-38%2fR228S&sid=ba085935cbae13802fd5ee2d9370eef5&sot=b&sdt=b&sl=111&s=TITLE-ABS-KEY%28Molecular+docking+analysis+and+determination+of+minimum+inhibition+concentration+of+VIM-38%2fR228S%29&relpos=0&citeCnt=0&searchTerm= | |
dc.description.abstract | Objective: The aim of this study was to determine the effects of residues at 228th positions of VIM-38 on minimum inhibition concentration (MIC) and molecular docking analysis. Materials and methods: blaVIM-38 gene was cloned into expression vector pET100/D-TOPO. Then pET100/D-TOPO-VIM-38 was used to generate the R228S mutation by site-directed mutagenesis. The mutant was transformed into E. coli Dh5α. Changes in the activity of mutation R228S was determined by E-test. Molecular docking analysis of the binding affinity of meropenem and imipenem with VIM-38 and VIM-38 R228S was performed using AutoDock Vina_1_1_2. Results: According to E-test results, pET100/D-TOPO-VIM-38 and pET100/D-TOPO-VIM-38/ R228S have same MIC value for amoxicillin, ticarcillin/clavulanic acid, ampicillin and ticarcillin. It was determined decrease in pET100/D-TOPO-VIM-38/ R228S of MIC for aztreonam, amikacin and cefixine. There are 3-fold deference in MIC between pET100/D-TOPO-VIM-38 and pET100/D-TOPO-VIM-38/ R228S for cefoxitin. pET100/D-TOPO-VIM-38 has 0.5 µg/ml and pET100/D-TOPO-VIM-38/ R228S has 0.125 µg/m for imipenem, we observed 4-fold deference. Also, there is about 170-fold deference between pET100/D-TOPO-VIM-38 (16 µg/ml) and pET100/D-TOPO-VIM-38/ R228S (0.094 µg/ml) for meropenem. VIM-38 and VIM-38 R228S have very close to the negative low free energies of binding of the meropenem and imipenem. Conclusion: These results showed that substitution (R228S) slightly have effect on enzyme activity. © 2017, Scientific Publishers of India. All rights reserved. | en_US |
dc.language.iso | eng | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Docking; MIC; Mutation; VIM | en_US |
dc.title | Molecular docking analysis and determination of minimum inhibition concentration of VIM-38/R228S. | en_US |
dc.type | article | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.department | Fakülteler, Mühendislik ve Doğa Bilimleri Fakültesi, Genetik ve Biyomühendislik Bölümü | en_US |
dc.contributor.institutionauthor | Özad Düzgün, Azer | |