Effect of olanzapine on complex 1, 2, 3 and ATP synthase

Abstract

Olanzapine belongs to the group of medicinal drugs called atypical antipsychotics. The primer antimanic and antipsychotic effect of olanzapine are likely regulated by the blockade of dopamine D2 and serotonin 5HT2A receptors particularly in the mesolimbic pathway in the brain. In addition, a distubance of energy metabolism or disfunction of mitochondria in patients with bipolar disorder and schizophrenia are observed frequently in these disorders. It is exactly unknown whether olanzapine may have theropeutic potential on mitochondrial respiration in the brain cells. For this reason, the present study aims at examining the effects of olanzapine on mitochondrial complex 1, 2, 3 and ATP-synthase in the brain right frontal cortex tissues of rats. Material and Method: In our study, 30 adult wistar-albino rats were used. Experimentally, rats were divided into two groups: control and olanzapine groups. Olanzapine and isotonic saline solution were administered intraperitoneally for 30 days. At the end of the experiment, the rats were decapitated. Brain right prefrontal cortices were taken into SET buffer (250 mM sukroz, 2 mM EDTA, 10 mM tris-base pH 7.4). Homogenization was then carried out in biochemistry laboratory. Supernatant fraction from homogenate containing mitochondria parts were taken for biochemical analyzes. Mitochondria complex 1, 2, 3 and ATP-synthase were measured by using kits and protein amount was measured by using Lowry method. Results: According to statistical analyse results, no statistical alteration was observed for studied parameters complexes 1, 2, 3 and ATP-synthase activities between the control and olanzapine groups. Conclusion: The present results show that olanzapine treatment does not lead to any changes in complexes 1, 2, 3 and ATP-synthase activity of the electron transport chain in brain tissues of rats.