| dc.contributor.author | Topal, Meryem | |
| dc.date.accessioned | 2014-09-15T12:45:03Z | |
| dc.date.available | 2014-09-15T12:45:03Z | |
| dc.date.issued | 2014-08-15 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12440/323 | |
| dc.description.abstract | Rosmarinic acid is a water-soluble ester of ca eic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of
rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) puri ed from human erythrocytes by using
Sepharose-4B a nity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold puri cation of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO2 -esterase activity gave Ki values of 86.0 M and 57.0 M, respectively. | en_US |
| dc.language.iso | eng | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Carbonic anhydrase I, carbonic anhydrase II, rosmarinic acid, enzyme puri cation, enzyme inhibition | en_US |
| dc.title | Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor | en_US |
| dc.type | article | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.department | Meslek Yüksekokulları, Gümüşhane Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü | en_US |
| dc.contributor.institutionauthor | Topal, Meryem | |
