Optimization of Different Surface Modifications for Binding of Tumor Cells in a Microfluidic Systems

Abstract

Objectives:Microfluidic technology is a fast-growing area and provide high-efficient MEMS (Micro-Electro-Mechanical-Systems) sensor integration platform that helps to advance healthcare systems. Due to proper the chemical and mechanical properties of polymers, PDMS (Polydimethylsiloxane) (6) and PMMA (Poly-methyl-methacrylate), they became on the best candidate for health care studies in microfluidic studies (7). Besides, they perform great optical properties for observation of living cell experiments. To increase their performance, surface interactions works with cells, modification techniques are widely used in microfluidic chips. In this paper, our primary purpose is to modify such polymers and glass with matrigel, PDA and APTES so as to increase cell-surface interaction. Patients and Methods:Cells were seeded into the micro-channels that is modified with 1%, 3% and 5% matrigel. The cell culture were observed 48-hours and images were taken. In the next stage, static culture experiments were performed on glass surfaces modified with PDA (polydopamine) and APTES (3-Aminopropyl triethoxysilane). 3-hour images were received. Results:The area filled by the cells was calculated using the ImageJ software ver.1.149. In the Matrigel modified chips, at the end of the 48th hour, the surface area of the cells reached to 22.58%-29.14- 26.97% for the matrigel sample rates 1%, 3% and 5%, respectively. In PDA, APTES modified surfaces (dissolved in ethanol) and APTES modified surface (dissolved in water), the cells overlaid on surface after 3rd hour 5,32% 8,08%; and 3,33%, respectively. Conclusion:The tendency of the cells to attach and colonize on increasing matrigel concentrations and on PDA, APTES modified surfaces was higher than the unmodified surface.